Scientists Use CRISPR Gene Editing Tool To Manipulate Behavior In Ants

This photograph shows Ooceraea biroi workers tagged with color dots for individual behavioral tracking. Credit: Daniel Kronauer The Rockefeller University

This GMO operation (using CRISPR technology) is permanently changing the germline of these ants and with the specific purpose of changing their social behavior. Technocrats do not consider ethics or unfavorable outcomes that will surely occur.  TN Editor

The gene-editing technology called CRISPR has revolutionized the way that the function of genes is studied. So far, CRISPR has been widely used to precisely modify single-celled organisms and, more importantly, specific types of cells within more complex organisms. Now, two independent teams of investigators are reporting that CRISPR has been used to manipulate ant eggs—leading to germline changes that occur in every cell of the adult animals throughout the entire ant colony. The papers appear August 10 in Cell.

“These studies are proof of principle that you can do genetics in ants,” says Daniel Kronauer, an assistant professor at The Rockefeller University and senior author of one of the studies. “If you’re interested in studying social behaviors and their genetic basis, ants are a good system. Now, we can knock out any gene that we think will influence social behavior and see its effects.”

Because they live in colonies that function like superorganisms, ants are also a valuable model for studying complex biological systems. But ant colonies have been difficult to grow and study in the lab because of the complexity of their life cycles.

The teams found a way to work around that, using two different species of ants. The Rockefeller team employed a species called clonal raider ants (Ooceraea biroi), which lacks queens in their colonies. Instead, single unfertilized eggs develop as clones, creating large numbers of ants that are genetically identical through parthogenesis. “This means that by using CRISPR to modify single eggs, we can quickly grow up colonies containing the gene mutation we want to study,” Kronauer says.

The other team, a collaboration between researchers at New York University and the NYU School of Medicine, Arizona State University, the Perelman School of Medicine at the University of Pennsylvania, and Vanderbilt University. , used Indian jumping ants (Harpegnathos saltator). “We chose this species because they have a peculiar feature that makes it easy to transform workers into queens,” says Claude Desplan, a Silver Professor at NYU and one of the senior authors of the second study. If the queen dies, the young worker ants will begin dueling for dominance. Eventually, one of them becomes a “pseudoqueen”—also called a gamergate—and is allowed to lay eggs.

“In the lab, we can inject any worker embryo to change its genetic makeup,” Desplan says. “We then convert the worker to a pseudoqueen, which can lay eggs, propagate the new genes, and spawn a new colony.”

Desplan, co-senior author Danny Reinberg, a Howard Hughes Medical Institute investigator at NYU Langone, and Shelley Berger, the Daniel S. Och University Professor in the departments of Cell and Developmental Biology and Biology at Penn, began studying these ants several years ago as a way to learn about epigenetics, which refers to changes in gene expression rather than changes in the genetic code itself. “The queens and the worker ants are genetically identical, essentially twin sisters, but they develop very differently,” Desplan says. “That makes them a good system for studying epigenetic control of development.”

The gene that both research teams knocked out with CRISPR is called orco (odorant receptor coreceptor). Ants have 350 genes for odorant receptors, a prohibitively large number to manage individually. But due to the unique biology of how the receptors work—a great stroke of luck, in this case—the investigators were able to block the function of all 350 with a single knockout. “Every one of these receptors needs to team up with the Orco coreceptor in order to be effective,” says Waring Trible, a student in Kronauer’s lab and the first author of the Rockefeller study.Once the gene was knocked out, the ants were effectively blind to the pheromone signals they normally use to communicate. Without those chemical cues, they become asocial, wandering out of the nest and failing to hunt for food.

Read full story here…

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